Super-resolution Imaging Technologies in Life Science
– An overview of the super-resolution imaging technologies in life science sector to evaluate cells at the nanoscale level
– Quantitative analysis of the market outlook for super-resolution imaging systems
– Discussion of various types of super-resolution imaging systems, configuration of these systems, their applications in biophysical investigations, and recent technological achievements
– Current technology assessment as well as outlining trends that are expected to contribute to market growth for these imaging technologies
– Comparison between three preeminent kinds of super-resolution microscopy technologies, such as stimulated emission depletion microscopy (STED), photoactivated localization microscopy (PALM) and stochastic optical reconstruction microscopy (STORM)
– Discovering the advantages and challenges in using these techniques together; along with current and emerging trends for improving super-resolution imaging systems
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Super-resolution imaging (SRI), which is also known as nanoscopy or super-resolution microscopy, is a group of technologies that allow to perform optical imaging beyond the diffraction limit of light. Light consists of electromagnetic radiations with wavelike characteristics. When light passes through a small opening or meets a small obstacle, it does not continue in a straight path, but it bends. This phenomenon is known as diffraction.
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Image resolution measures the amount of details in an image. The resolution of optical instruments, such as microscopes and telescopes, is affected by diffraction. The diffraction limit is the minimum distance between two objects that permits to differentiate the objects one from the other. This limit was defined in 1873 by the German physicist Ernst Abbe as: d=?/(2n sin?)=0.5 ?/NA, where d is the diffraction limit, ? is the wavelength of light, n is the index of refraction of the transmitting medium, and ? is half the aperture angle of the light. In an optical instrument, NA= n sin ? is the numerical aperture of the objective lens that collects the light.
Table of Contents
Chapter 1 Technology Highlights and Market Outlook
Applications of Super-resolution Imaging in the Life Sciences
Types of Super-resolution Imaging Systems
Fluorescence and Epifluorescence Microscopes
Super-resolution Imaging Systems
Current and Emerging Trends
Advanced Multicolor Systems
Image Quality Improvements
Faster and High-Throughput Systems
Recent Achievements in the Life Sciences due to Super- resolution Imaging
Market Outlook for Super-resolution Imaging Systems in the Life Sciences
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